Understanding NAD+

Nicotinamide Adenine Dinucleotide (NAD+) is a coenzyme found in all living cells. It plays a critical role in energy metabolism, DNA repair, and cellular signaling.

Why NAD+ Declines with Age

NAD+ levels decrease by approximately 50% between ages 40 and 60 due to:

• Increased consumption by DNA repair enzymes
• Activation of CD38 (an NAD+-consuming enzyme)
• Reduced biosynthesis capacity
• Chronic inflammation

Key Functions of NAD+

Energy Production

NAD+ is essential for mitochondrial function:

• Facilitates ATP synthesis
• Supports cellular respiration
• Enhances metabolic efficiency

DNA Repair

Activates sirtuins and PARPs:

• Repairs damaged DNA
• Maintains genomic stability
• Reduces cellular senescence

Circadian Rhythm Regulation

Influences sleep-wake cycles:

• Regulates CLOCK genes
• Optimizes metabolic timing
• Improves sleep quality

Benefits of NAD+ Supplementation

Cognitive Enhancement

• Improved mental clarity and focus
• Enhanced memory formation
• Neuroprotection against age-related decline
• Better stress resilience

Physical Performance

• Increased endurance and stamina
• Faster recovery from exercise
• Enhanced muscle function
• Improved cardiovascular health

Metabolic Optimization

• Better insulin sensitivity
• Enhanced fat oxidation
• Improved glucose metabolism
• Increased metabolic rate

Administration Protocols

IV/Injection

Most effective delivery method:

• Dose: 500-1000mg per session
• Frequency: 1-2 times per week
• Onset: Immediate effects
• Duration: 3-7 days

Sublingual

Good bioavailability:

• Dose: 50-100mg daily
• Timing: Morning on empty stomach
• Absorption: 40-60%

Synergistic Combinations

NAD+ Boosting Stack

• NMN or NR: NAD+ precursors
• Resveratrol: Activates sirtuins
• Pterostilbene: Enhanced bioavailability
• Quercetin: CD38 inhibitor

Mitochondrial Support

• CoQ10: Electron transport chain
• PQQ: Mitochondrial biogenesis
• Alpha-lipoic acid: Antioxidant support

Expected Results

Timeline for noticeable benefits:

• 1-3 days: Increased energy, mental clarity
• 1-2 weeks: Improved sleep, mood enhancement
• 4-8 weeks: Physical performance gains
• 3-6 months: Anti-aging effects, metabolic improvements

Safety Considerations

• Generally well-tolerated
• Mild flushing possible (histamine response)
• Start with lower doses to assess tolerance
• Consult healthcare provider if on medications